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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have been described. Two more splice variants were being noted but in depth nucleotide sequence info isn't but readily available.
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Blocking peptides are peptides that bind specifically towards the concentrate on antibody and block antibody binding. These peptide generally consists of the epitope recognized through the antibody.
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This guide outlines more probable optimization of mobile and immunoassay parameters to make certain the very best outcomes are...
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This manual outlines even further doable optimization of mobile and immunoassay parameters to guarantee the very best success are...
Antibodies sure to the blocking peptide now not bind to your epitope to the focus on protein. By evaluating the staining through the blocked antibody compared to the read more antibody on your own, you can see which staining is unique.
Goal Details Cyclin E belongs towards the extremely conserved cyclin family, whose users are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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